I don’t know that, but I’m pretty sure a Y-chromosome match was involved using a living descendant and the Richard III skeleton (“The King in the Car Park”). Now, it’s not just a matter of the Y being resistant to change, but the rate of change being known, but at any rate, a 500 year gap and a non-direct descendant apparently was good enough. There was probably more to it than just the Y-chromosome, though.
Side note: I REALLY wish they’d compare the Ys on those boy skeletons buried in Westminster as the Princes in the Tower with the Richard DNA. The last time those skeletons were examined was the 1930s, before DNA was discovered.