•Interrogation Chamber.
The layout of which can vary, depending on the morals and ethics of your team. (i.e., whether they’re willing to kill or maim a villain.) You may want to include interrogation equipment and methods that won’t actually cause harm, or you might want to be set up to perform outright torture. The CIA’s Interrogation Manual might be of some help for outfitting such an area in your base.
You might use real-world interrogation equipment—Sodium Pentothal; sensory deprivation chamber(s); a strappado, etc.—or you might be able to use more science fictiony devices, if they’re available in your heroes’ universe—truth rays; agonizers; hypno-rays; a telepath, etc.
•A Crematorium.
Even if you don’t set out to kill your enemies…if they do end up dead, you should probably incinerate the bodies as thouroughly as possible, to keep them from rising from the dead very easily (if at all). If your base uses some sort of nuclear fusion as a power source, you might use reactor plasma instead of natural gas to burn the body. (Unless the villain gets his/her power from high energy plasma, or something.)
In fact…you should probably make provisions the shoot the body in the brain, dismember it, dunk it in a vat of acid, and then burn it. You can never be too safe.
•A Deep-Freeze Storage Bunker.
At the very least, it should be a secure freezer for storing tissue/DNA samples; viruses and bacteria; semen and ova, etc. But, if your universe allows it, you might have a setup to cryonically store whole human bodies for future revival. (Or just as a secure form of detention.)
•A chamber that can suspend things in a magnetic field, in a vaccum chamber.
This’ll come in handy, sooner or later.
•At least one room shielded against telepath, magic, and/or listening devices.
Just keep a log of who enters the room, and when.
•A section of the Medlab that has tall, glass, liquid-filled tubes for keeping bodies in.
They could be life-support tubes for growing clones, treating severe injuries, etc. Or you might fill them with alcohol or formaldehyde, to store corpses in. Or you could make 'em into huge fish tanks, to brighten up the place.
•A specially reinforced chamber for detonating explosives in/defusing bombs in.
You can probably use it as a water tank or something, when you aren’t using it for bombs.
•Private movie theater.
Can double as a briefing hall.
•An escape tunnel linking into the NYC subway systems. Maybe you can even use a fake subway car, for a covert exit.
•A hyperbaric chamber.
Attached to the sickbay/medlab. Can also be used for interrogation, a la Tom Clancy.
Of course, you’ll need some sort of secondary backup base, in case the main base is compromised or destroyed. You can probably put it somewhere out of the way, like the Catskills, or Newark. You can probably have a bit of fun with designing the backup base by having it be basly outdated, and poorly maintained. (Like filling it with really old computer equipment, or something.)
And remember to take some tips from the pros.