Poll coming. Yes, I always use a shot glass when I make cocktails at home and it kinda bugs me when people don’t, there are several on my bar cart. The only time I don’t is during the summer if I’m making frozen drinks in the blender where I need to use a measuring cup to make a pitcher of margaritas or daiquiris.
Do you mean: “do you measure cocktails” or is the question specifically about the glass?
I measure for (most) cocktails, but I do so with a jigger, not a shot glass. I don’t think I own any shot glasses anymore. I believe they all got broken during parties during my 20s (the proper viking-funeral end to a shot glass) and I just never bothered to replace them.
Pretty much any attempt to measure, if you’ve worked in a bar and know how to pour one shot without using a shot glass, then that’s fine, that counts as use a shot glass.
Always (jigger). We use a particular set of cocktails glasses, and measuring makes sure I don’t make too little cocktail for the glass. That’s only for the wife’s martini and my Manhattan, however. If we make Margaritas it’s pour by eye. Into a glass with ice. Frozen Margaritas are an Abomination Unto Nuggan.
Depends. If I’m mixing a drink that matters, like a margarita, which honestly doesn’t happen that often then I will. Or mojitios (we have a ton of mint).
If I’m making what most often passes for a mixed drink around here, which is vodka-and-something, I will for my wife’s, not mine. She likes hers with exactly a shot. I tend to pour mine a little heavier.
I’ve been drinking captain and cokes and mojitos at home lately. I always hand pour. I leave room at the top to dilute, my drinks are usually strong.
We don’t need no steenkin’ shot glass.
We have one of these with fluid ounces, teaspoons, tablespoons, portions of a cup, etc all marked on it.
ETA: and metric, too
I voted hand-pour, but then read your later post that hand-pouring counts as using a shot glass if you know how much you’re pouring. Now I don’t know what I was supposed to vote on.
I use a jigger.
The wife hand-pours my Bloody Marys, but she knows I like them “see-through” so it isn’t a problem.
It depends. Usually not, but sometimes. If I’m making mojitos, for instance, I’m very precise about everything except the club soda. (Not that you can really be all that precise about how much juice comes out of any given lime.) I have a couple of different mojito recipes and I think if it was just a plain mojito I would probably just eyeball it, but for the others, I measure…
But my fave summer cocktails are those mixers you buy in big cartons and then pour a quart of the right booze into and put into the freezer, and then scoop it out as needed once it’s frozen, so all you gotta do is put salt on the rim of the glass.
Nope. Eyeball it or count the length of the pour. I know how I like my drinks to taste, so it’s not necessary.
No I do not.
Further, I do not need to.
No, and I’ve never worked in a bar nor do I really care. I drink rarely and throw stuff in there and drink it.
Never used to. Of course, my go to cocktail was gin over ice in a glass. Maybe that’s part of the reason why I decided to stop drinking?! 
A while back my wife got started on the use of measuring cocktail ingredients, observing that both of us tended to make drinks WAY too strong. Once we ceased being irresponsible young people and became slightly less irresponsible older people, we realized the point WASN’T necessarily to get everyone as fucked up as possible as quickly as possible. Who woulda thought?
Nope, I drink brown booze and clear mixer, so I pour based on color and strength preference. Besides, what good is measuring the booze if you don’t measure the mixer? I never tended bar for a living, but I did grow up in Wisconsin.
You do measure all the ingredients.
It depends. I was a bartender for some years in my younger days and can hand pour a fairly accurate shot without measuring, only a little generous. For my own drinks, I always free pour because I know how I like them.
But there are exceptions, even for my own drinks. I always measure when I’m making a new cocktail so I know how it’s supposed to turn out. After that, I adjust to my own taste. And some things require precise measurements – the perfect martini springs to mind. I use a 5-1 gin-to-vermouth ratio, so measuring matters.
I don’t use a jigger, but I do measure with a 1/4 cup for a shot for most of the drinks I make.
Who am I kidding…I use a 1/3 cup for measuring. 
I used to bartend so I have proper pourers on my booze. So yes, I measure a three count, then add more to cater to my alcoholic tastes.