I think I can come up with a few:
- What counts as a “person”, and deserving of rights? An AI? A partially-uplifted animal? An alien?
A.I: 2001, Blade Runner, Bicentennial Man, Short Circuit, A.I.: Artificial Intelligence, iRobot, Her, Ex Machina, Chappie, Blade Runner 2049, Archive
Aliens: E.T., Starman, The Abyss, District 9, Avatar
- Is it ethical to duplicate a person? Having done so, what is it acceptable to do with the duplicate?
Multiplicity, Moon, The Island, Never Let Me Go, Upload, Transcendence, Replicas
- Is it acceptable to change the past? Is it acceptable to not change the past? Does the answer change if there’s reason to believe that you can’t succeed?
The Time Machine, The Butterfly Effect, About Time, Looper, Hot Tub Time Machine, Tenet
- Is it acceptable to punish someone for something they haven’t done yet, but which you know they will? Does it depend on how you know-- Is it just through advanced psychology, or through actual precognizance, time travel, or the like?
Minority Report is the main one for precognition. Gattaca for genetic profiling.
- Are alien cultures bound by human ethical standards? Should they be (if that’s even a different question)?
Frequently comes up in Star Trek and Doctor Who. Less common than the other themes as humans are not typically presented as being in a position to hold alien cultures to our standards.
An offshoot of that might be is it ok to commit genocide to ensure humanities survival (Ender’s Game, Star Trek)