Vaguely familar.
Will you remind me where it came from? (I’m tired.)
Star Trek: TOS. The one where some aliens steal Spock’s brain, IIRC. The actual line is “Brain and brain what is brain?”
I can’t tell you much about specific nutrients you’ll need, but here is how to make a clean room for under 20 bucks:
- You’ll need a closet and a towel hanger.
Attach a towel hanger to the wall in your room, take all the clothes out of the closet and put them on the new hanger, remove the old one. Now you’ve got an empty closet. Good. - Go to a supermarket, get large garbage bags, scotch tape, pins, household bleach and a spray bottle.
- Now this is hard part - and will take a few hours. You need to cover all the walls with garbage bags, attaching them to the wall with pins, and seal them with scotch tape. Entrance to the closet should also be covered with plastic, so no air goes in or out.
- Wipe the plastic on inside with a sponge with hydrogen peroxide, let it dry, then do the same with rubbing alcohol. Let it dry.
- Make a 10% bleach solution in boiled water (does not have to be sterile). Put one hand into the clean room, spray it around, close it and leave for 10 minutes.
- Furniture. You’ll need to find a plastic chair, a steady table (better be plastic) and a table top lamp. Wipe them with H2O2 and rubbing alcohol, put them into clean room, spray 10% bleach in the same way as before.
- Before going in - take a shower, put on clean lab coat and clean pants (coat and pants should be either kept in the clean room, or clean box, or washed every time). Wear a utility mask over your face and a plastic bag over your hair. Gloves are optional. Hands must be clean.
- Spray 10% bleach solution every day, but always give it a few minutes to settle before going in, you don’t want to breathe that.
- For ventilation you can attach an old fan to a HEPA filter from Can Tire, installed into a hole in the plastic.
- For heating - get a $5 aquarium heater, fill up any 2-3 L container with water and just stick it in. Pepsi bottle will do.
For even better results - you can keep a large cooler in the clean room, and incubate your petri dishes in there, it provides better insulation for light and heat - so you won’t freak out next time electricity goes out + you get a higher degree of protection against bacteria, mold or friends.
Maybe I’ve been watching too much Terminator: Sarah Conner Chronicles on NetFlix recently, but all I can envision is the OP accidentally creating SkyNet in his garage. It would be truly ironic to have humanity exterminated by rat brain cells.
Oh no!
Zombie Brains in a Petri dish!
Thankfully, without the plague to help them rats aren’t good mass murderers. They have neither the time, the training nor the inclination for strategic thought. Now, if the OP was trying to grow Hitler’s brain in a jar…
Pinky & The Brain: The Prequel.
But seriously, could you please pick a species with less reason to detest humanity? Or at least make sure there aren’t any open wireless networks around? 