My first and only encounter with that was Jayne on Firefly. Other than, you know, people repeating it in forums, in which case I never got an impression that they weren’t quoting Jayne.
ETA: Wow I’m surprised to see that it was the first reference. I’m not sure whether to be glad (Joss Whedon is a relatively original writer) or disappointed (you made me think Joss was referencing some famous earlier quote).
I had no idea it was a pop-culture reference at all. I’m actually a little disappointed (but not much). I think it’s funny because it’s a phrase that in a literal sense is so innocuous and yet the salaciousness is immediately obvious, not because it’s from a TV show (of course explaining things in big words always kills the funny).
But perhaps for the benefit of those who abhor this obnoxious SDMB catchphrase, we can come up with something less offensive, like “hold on while I rub one out” or “pardon me while I manipulate my penis until ejaculation results.”
I can’t believe everyone is attributing this to a recent TV series. Every guy was saying this at the college I went to a couple decades ago. The typical pronunciation was: “Ah be in mah bunk.” I have no idea what the accent was supposed to be – kind of ghetto, I guess. But some guys made it sound more like Ahh-nold. I don’t like to think of the Terminator pleasuring himself. What kind of a devastating shot must that be? You’ll put your eye out!