What I do is put a metal spoon in hot water to heat up the spoon and then apply it to the bite. Using two spoons is quicker since I can alternate with one on the bite and one in the water heating. After about 5 or so applications of the hot spoon, the itch is gone and doesn’t come back. I’ve also used pen devices which apply heat, and they work as well.
Yeah you can use anything that’s hot. But hot water is free 
The heat should be hot enough to be uncomfortable but NOT hot enough to burn you.
Perhaps @mozchron can explain my mosquito experience. For my first 18 years I reacted to mosquitos the same away most people do. I got bitten and they itched for a couple days. Then I got a job in a biology lab, working with a bunch of chemicals of which likely the most notable was amyl acetate. As long as I worked in that lab (4 years) I was never bitten. Since then I am bitten occasionally, but the bites do not swell and do not itch.
This is also believed to be one way people can develop allergies to beef and pork.
I’ve used ice, which also works, and hydrocortisone (or another steroid) cream does the job as well.
… as far as you are aware.
I don’t react to mosquito bites much of the time.
I have a tiny home office in my backyard that is fully screened, but occasionally a mosquito gets in when I open the door. Yesterday a mosquito was whining around in front of my computer monitors so I could see it very clearly. It was not blood engorged when I first saw it. I failed despite several attempts to kill it but then it disappeared. 10 minutes later it reappeared, and was blood engorged. Unquestionably it had bitten me in the meantime but there were no indications of that from my perspective.
My money would be on the mosquito having bitten you or your family without your knowledge.
could be. Also, if what @mozchron says is correct, it is possible that it was from a bite I got two days earlier (I think I did get bitten then). Could be either.
out of curiosity, what do you use the DNA for?
I think that (Alpha-gal reaction) is more a feature of tick bites than mosquito bites, I don’t recall hearing about that with skeeters.
You will generally get used to bites over time and stop reacting/react less (unless you get hypersensitive and then it’s WAY worse). I have no explanation about your chemical lab job.
No, that’s a reaction to (certain) tick bites. Alpha-Gal syndrome.
Many, many things. To screen for pathogens (we might extract RNA in this case depending on what we’re looking for). To sequence the genome. We do a ton of genetics and transgenic works, so we extract DNA to confirm genetic edits or to genotype mosquitoes. And a million other things; it’s our bread and butter.
The bloodmeal DNA extractions were/are used to identify pathogens in the bloodmeal, genotype those pathogens, identify the species that the mosquito fed on, or even identify the people the mosquito fed on.
is it used for police forensics?
I think that would drive me bananas
Funny you should ask that. When we started this (back when I was a wee graduate student) we used the same kits the FBI used to genotype blood from crime scenes.
To answer, yes it’s happened. It’s not super common but it has been done. Here is a paper describing one such example:
Actually, it gave me headaches, but it did lead to an aversion to bananas, which has fortunately gone away.
I beleive you were also working on some cool stuff with genetics for mosquito population control? Something about breeding sterile males, I think, and releasing them in the wild to mate with the females, who also produce sterile males? You shared this here but I cannot find the thread. Any progress on that project?
Fascinating stuff, BTW!
Same here, I used to react until sometime in my mid-teens but for the last 40 years or so I don’t react to the bites. Oddly enough, I am allergic to trees, pollen, grass etc. with hay fever so there is no histamine shortage in my body from other causes.
Would it be possible to get mosquito shots to build up a tolerance to bites in the same way that people get allergy shots to build up a tolerance to common allergens?
Probably but there are many, many different species of mosquitoes; you’d have to do all of them. So probably not logistically feasible.