Ok, this is a fairly high-level question, but the textbook I’m working with seems to be buggy in this area, so I figured I’d see if there are any transposition expert dopers out there. I won’t be at all surprised if this question goes unanswered, but I figure it’s worth a shot (before heading down to one of the UT bookstores and seeing if there are any other texts that explain this satisfactorally
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Ok, during replicative transposition, the transposon invades the target DNA, resulting in a doubly branched DNA molecule, with the transposon in the middle, the target DNA bound at the 3’ ends of the transposon (with the other strand of the target DNA cut), and the host DNA bound at the 5’ ends of the transposon)
The transposon DNA is then replicated, starting at one or both ends, and using the 3’ ends of the target DNA (the cut strands) as a primer. As far as I can model it, this results in two DNA molecules, each consisting of one half of the host DNA, the transposon, and one half of the target DNA. But I’m pretty sure it’s SUPPOSED to end up with the transposon as it was before any of this started, plus a copy of the transposon inserted into the target DNA.
Am I missing something? Am I right up until my assumption about what you’re SUPPOSED to get? I could just wait for the next version of the art (I’m designing an animation to explain this process), but I’d like to get this finished before then 