There are 20 possible first moves. The number of second moves varies according to the first move played:
If 1. a3, then 2. a4, b3, b4, c3, c4, d3, d4, e3, e4, f3, f4, g3, g4, h3, h4, Ne3, Nf3, Nh3 (18 second moves)
If 1. a4, then you can’t play 2. a4, but you can play 2. Na3 (18 second moves)
If 1. b3, then 2. a3, a4, b4, c3, c4, d3, d4, e3, e4, f3, f4, g3, g4, h3, h4, Na3, Nc3, Nf3, Nh3, Bb2, Ba3 (21 second moves)
If 1. b4, then you can’t play 2. b4, and nothing else changes (20 second moves)
If 1. c3, then 2. a3, a4, b3, b4, c4, d3, d4, e3, e4, f3, f4, g3, g4, h3, h4, Na3, Nf3, Nh3, Qc2, Qb3, Qa4 (21 second moves)
If 1. c4, then you can’t play 2. c4, but you can play 2. Nc3 (21 second moves)
If 1. d3, then 2. a3, a4, b3, b4, c3, c4, d4, e3, e4, f3, f4, g3, g4, h3, h4, Na3, Nc3, Nd2, Nf3, Nh3, Bd2, Be3, Bf4, Bg5, Bh6, Qd2, Kd2 (27 second moves)
If 1. d4, then you can’t play 2. d4, but you can play 2. Qd3 (27 second moves)
If 1. e3, then 2. a3, a4, b3, b4, c3, c4, d3, d4, e4, f3, f4, g3, g4, h3, h4, Na3, Nc3, Ne2, Nf3, Nh3, Be2, Bd3, Bc4, Bb5, Ba6, Qe2, Qf3, Qg4, Qh5, Ke2 (30 second moves)
If 1. e4, then you can’t play 2. e4, and nothing else changes (29 second moves)
If 1. f3, then 2. a3, a4, b3, b4, c3, c4, d3, d4, e3, e4, f4, g3, g4, h3, h4, Na3, Nc3, Nh3, Kf2 (19 second moves)
If 1. f4, then you can’t play 2. f4, but you can play 2. Nf3 (19 second moves)
If 1. g3, then 2. a3, a4, b3, b4, c3, c4, d3, d4, e3, e4, f3, f4, g4, h3, h4, Na3, Nc3, Nf3, Nh3, Bg2, Bh3 (21 second moves)
If 1. g4, then you can’t play 2. g4, and nothing else changes (20 second moves)
If 1. h3, then 2. a3, a4, b3, b4, c3, c4, d3, d4, e3, e4, f3, f4, g3, g4, h4, Na3, Nc3, Nf3 (18 second moves)
If 1. h4, then you can’t play 2. h4, but you can play 2. Nh3 (18 second moves)
If 1. Na3, then 2. b3, b4, c3, c4, d3, d4, e3, e4, f3, f4, g3, g4, h3, h4, Nf3, Nh3, Nb5, Nc4, Nb1 (19 second moves)
If 1. Nc3, then 2. a3, a4, b3, b4, d3, d4, e3, e4, f3, f4, g3, g4, h3, h4, Nf3, Nh3, Na4, Nb5, Nd5, Ne4, Nb1 (21 second moves)
If 1. Nf3, then 2. a3, a4, b3, b4, c3, c4, d3, d4, e3, e4, g3, g4, h3, h4, Na3, Nc3, Nd4, Ne5, Ng5, Nh4, Ng1 (21 second moves)
If 1. Nh3, then a3, a4, b3, b4, c3, c4, d3, d4, e3, e4, f3, f4, g3, g4, Na3, Nc3, Nf4, Ng5, Ng1 (19 second moves)
So, there are 20 + 18 + 18 + 21 + 20 + 21 + 21 + 27 + 27 + 30 + 29 + 19 + 19 + 21 + 20 + 18 + 18 + 19 + 21 + 21 + 19 = 447 possible first two moves for White if Black does not move.