For whatever reason, amputations, especially for laughs, make me really uncomfortable.
There’s a story arc in Modern Family where this trashy family moves in next door to the Dunphys. The teenage son is trying to hit on the neighbor’s daughter, who is clearly out of his league. They have this little exchange where it looks like she might be warming up and then she says “go back home to your mommy, you little bitch”. Then they just show his stunned, hurt face and the scene ends. I must have seen this episode a half dozen times and it always takes me aback. Feels really harsh for a sitcom.
Lots of comedies promise mayhem. Some just decided to use the legal definition. 
You definitely should not watch Monty Python and the Holy Grail, which also has an amputation scene that could qualify it for this thread. Also gratuitous and violent death scenes.
I don’t want to speak for @Dewey_Finn, but I don’t think “cartoon violence” (which isn’t limited to actual cartoons; I think Holy Grail counts) has the same effect.
It’s been years (perhaps decades) since I saw Monty Python and the Holy Grail but as I remember, the amputation scene was cartoonish and comical and didn’t make me uncomfortable in the way other such scenes have.
What We Do in the Shadows is very, very funny and very, very dark. Last season one episode was about a ‘Home Improvement’ show doing an episode about their home, and the first thing they do is murder one of the hosts. It’s played for laughs. Regular humans die in all kinds of ways on that show.
In another episode there was a funny sequence where they kept manipulating the memory of someone until they killed him by accident. In other episodes they’ll have funny banter at the table, the main course being the corpse of a dead human.
I love that show, but that was one death that kind of got to me. Then they hypnotize the other guy so he doesn’t know his brother’s dead. Dark even for WWDITS.
Four Rooms has a dead hooker and an amputation played for laughs.
Fargo has several murders, and a corpse being fed into a wood-chipper.
There are a lot of fish-out-of-water comedies that use being on the run or the like to get to the situation. Then they get found in act three and the movie takes a hard turn to violence. Sister Act and Kindergarten Cop come to mind, i know they’re others.
My mother-in-law, who hates violence and gore in movies, loved the Black Knight scene. Go figure.
Did you watch it all the way to the end? It turned out that Bran was really the vampire Simon the Devious all along.
The only death that bugged me (just a bit) was at the wrestling match in Vegas.
I always think an amputation as more a medical procedure, as opposed to having your limbs hacked off by an opponent, be it cartoonish or not. IMHO YMMV
Yeah, he was, but he was also fooling the other brother, the normal human who was still killed at the beginning of the show if I recall correctly. And didn’t Simon kill the brother he replaced? That would makemit even darker.
I kept hearing ‘oh, you must see Fargo, it is hilarious, it’s a real classic!’ … I did, and I thought it was repellant. I liked the mildly awkward comical bits, but frankly, I found it gross. (same for Pulp Fiction. Gross.)
Funny, it’s been awhile since I watched it, but I do not remember a toe-losing scene in that movie. Musta blocked it out from my memory 
Yeah, same here- that scene was so silly and over-the-top, it was not in the least uncomfortable.
I had no problem with anything in Fargo. If I expect a movie to be violent or a dark comedy with violence, it’s not a problem. It’s when an otherwise relatively light-hearted comedy scene (even in what could overall be considered a ‘dark’ comedy) takes a left turn and somebody who is not a ‘bad guy’ is permanently injured that I go WTF.
It’s there, but they don’t show the deed, just the severed toe and later the foot with the wound where the toe was. That’s why we don’t know for most of the film who the toe belonged to.
ETA: yes, like @Miller said, the toe belonged to the female German nihilist and not to Big Lebowskis’ “kidnapped” trophy wife.
One of the nihilists cuts off her little toe and mails it to the Big Lebowski, as “proof” that they’ve kidnapped his wife and are carving bits off of her until he pays the ransom.
They don’t show the amputation, but the BL does hand the Dude the toe in an envelope, and later on you see one of the nihilists with a bandaged foot.
Oh yeah, I remember that now, of course. Thanks a lot for making me relive the trauma! 
Haha, that didn’t actually bother me. As mentioned, it’s only in certain scenes where it happens in scene, unexpectedly, to a sympathetic character; it just brings the comedy to a screeching halt.
Maude tackled some controversial subjects. Most notably the two-parter in season one when she gets pregnant, and ultimately has an abortion. It’s quite emotional.
