Yes it's about work again.

ERR… ERR

STOP FLASHING AT ME! AARRRRG stop it! STOP IT NOW!

I’ve had enough with all of you assholes, I’m gonna take a week off an re-evaluate my life’s goals!

Specifically, I’m going to raise a mutated monkey army.

Oh yes it will be mine! Carefully trained attack monkeys with the glow-in-the-dark sex organs virus and genetically engineered to explode easilly. I will unleash my army upon your buisnesses, I will send HIV infected chimps into your offices, lock the doors behind them and pipe chimp phermones in through the AC ducts.

In short: The gloves are off and I will OWN you!

I’ve been good long enough, but I just can’t take it anymore. If I practiced the level of incompetence that the contractors which work on this facility practice Texas would be a BURNING BIOLOGICAL WASTELAND.

Ok ok, it’s already a burning biological wasteland, but I bet ya I can make it worse!

So I’m thinking that if I’m going to become a crazed super villian/scientist I need a sexy underling. Ya know someone who can seduce the hero into making a fatal mistake and keep the monkeys in line.

<Competative wages, good health plan, leather body suit and riding crop provided. Perks include playing with monkeys and beating peons>

Ok: So here’ whats going on that’s annoying me.

18 ohm water.

The ultra pure stuff. Basically just H2O with no nasties in it. Reagent grade water, usefull for all sorts of biological experiments.

Our nanopure machine is all FUCKED up.

The probe which tells us what ohm it is (I have no clue what this means, all I know is that when the read out says 18.3 ohms I dispense water) is 12 years old, is shitty and keeps falling out of alignment. Which means the machine just flashes ERR ERR ERR

Over and over again.

ERR

Actually it’s more like Err see it can’t do the BIG r… only the little r…

It’s amaizing the amount of pH controled water we need to go through, water without contaminates which can disrupt DNA or contaminate cell culture conditions… But guess what:

THE FREAKING MACHINE DOESN"T WORK!

It hasn’t worked in 10 goddamn monkey raping months!

It’s either been leaking from the remote or the filter cartrages or the probe has been FUCKED.

In the last month I’ve had 4 guys come out to fix it.

“The probe is bad, but they don’t make em any more I might be able to get one for 2,000$+”

URK

“I can try wiping it off with ethanol…”

Then later, “the probe is out of alignment…”

After that’s fixed it starts leaking.

Then it takes 3 different people to realize the O-ring on the hand set that keeps the water from oozing out is bad!

But guess what:

THEY DON"T MAKE THE GODDAMN O RING! A 40 cent piece of plastic that NOBODY MAKES! AAAAAAAAAAAARRRRRRRRRRRRGGGGGGGGG

In the mean time: every few hours we have to mop of the water. Well by me I mean I. NOBODY ELSE NOTICES IT!

I leave the damn handset in the sink, not hanging on the hook over the counter so the water DRAINS INTO THE SINK!

So I played phone tag for 3 hrs today. Upshot of the whole thing, 480$ for a new handset. 65$ for a handset filter + labor for installing a new handset on a machine which may not accept it.

And lets not forget that they’ll probably leave something not connected so it’ll leak again.

I swear to christ the next repair man who fucks up fixing something gets his keys thrown in the breeding corral! Let’s see that fucker leave when he has to fight 100 lb baboons in heat!

What really is pissing me off is this:

Boss: Wants nanopure machine fixed, tired of leaks.
Grants Mangement: They want to know how much this is all going to cost so they can file their paperwork.
Repair guys: Want to get a work order BEFORE they bill us so they can make sure they get paid.
Accounting: Doesn’t want any repairs to go over 200$
Me: To be left alone.

To make matters worse: NOBODY KNOWS WHO PAYS FOR THIS SHIT!

My boss doesn’t want to pay since it’s not his. He didn’t buy it, the foundation provided it…
So someone somewhere, in a secret bunker (the same place where they hid the wet bar for the christmas party) knows who is responsible for paying for it, and or buying a replacement.

And everyone is calling me wanting to know what’s going on!
I"M A GODDAMN LAB TECHNICIAN!

HOW THE SWEETLY BATTERED CHRIST AM I SUPPOSED TO KNOW WHAT THE FUCK IS GOING ON!?

I don’t know if I can order a new one. I can’t authorize any repair which costs more than a can of coke…

AND NOBODY WHO ASKES ME WHATS GOING ON KNOWS HOW TO TALK TO EITHER!

OMFG. Everyone says, ‘it’s not my problem’.

Yes it fucking is!

Boss: I can live with it leaking.
Foundation: You’ll be the ones getting the fines for too much water use.
Accounting: You guys have to pay the piper so to speak
Grants Mangement: You’ll know how much it costs to fix AFTER WE GET IT FIXED!
Repair guys: You’ll get paid AFTER YOU FIX THE FUCKER RIGHT

So why is it out of all the people involved the one with the most daily work (me) has to take care of this?

I am scientific support staff… I support the scientists. You guys are support staff, you support the scientists.
Arrg

It’s not like this is a single incident.

The nanopure machine doesn’t work… some idiot forgot to replace the CO2 tanks for the central CO2 system (our tissue culture incubators need 5% CO2 to keep the cells alive… if the central CO2 system goes down the cells don’t get enough CO2 and they die). That caused a week of me running around checking pressures of backup tanks and hounding the work crew guys to install new holders for CO2 tanks… (that resulted in some guy using a screw driver to punch a 10 inch hole in the wall of the BSL-3 so he could get a starter hold for a screw going…)

We had freezer problems and the guy fixing it electrocuted himself on the floor of the lab.

We’ve had flooding, insect problems and god knows what else.

AAAAARRRRRRGGG

pat pat pat

It’s okay, man. Breathe deeply and evenly.

Yep,it sucks, but just think, it could be worse…you could be working as tech support for Micro$oft…

Where the hell are you CRorex? Your lab is fucked up!

I was at Baylor School of Medicine in the Medical Center, and I didn’t even HAVE a water supply in my lab. We would go down to another lab and fill our container with their RODI and use that. It took forever, but at least I wasn’t responsible, and it didn’t come out of my grant.

It did suck that one time I forgot to go back for it and left the water on and flooded his entire lab. Oops.

Can’t you just shut it off and scavenge from another lab until you get it fixed?

Am I the only person who thought of Tom Arnold in Austin Powers?

Easy man, you’re gonna blow an o-ring…drop a lung!

Just go and get a Biocell from Millipore (you can go through Fisher)… they are about 5K but they are nice and will give you that good old 18.2 along with DNA/RNAes free H2O… completly sterile, don’t even need to autoclave the crap. :smiley: If you have a crap DI system like we do here at WashU then I would suggest getting an Elix along with it but get one with a big reservour, it’s a RO deionizer that produces 15 MOhm from Tap H2O.

Just a sugestion, I feel your pain… we replaced a 20 year old Milli-Q system with what is described above.

Nicholas

I’m just popping in to apply for the assistant position. I come with my own leathers (available in pant and skirt form) and riding crop, but those health care benefits had better be good.

Where should I send my application?

I’d like to apply for the monkey training position. My qualifications include chasing after a four-year-old, experience in teaching high school, and an autoimmune disease easily transmitted through human plasma to make your monkeys even more dangerous. Must make monkeys more dangerous.

OOOOOOOOOOOOOOOOOOO!

VERY VERY NICE

<drools>

Hrrm do you know the type of system you guys have? Like the part number? I know we’ve got a good deal with fisher… and 5k isn’t all that expensive, I know my boss would like it… the only kicker would be convincing the foundation to buy it.

How well is it holding up?

I’d like to apply for the “sexy underling” position.
My qualifications :

I’m a redhead (nuff said).

I like monkeys.

I’m a hockey player. (I know how to cause pain and have experience in causing it).

Years of retail/customer service jobs have sharpened my anger to a razor’s edge.

I have my own riding crop, as well as hockey sticks and an aluminum baseball bat.

Being a wrestling fan as well as a hockey player, it’s a simple conclusion to make. I like the sight of blood.

:: Pours CR one o’ them frou-frou drinks with wedges of citrus fruit stuck in the rim of the glass, an umbrella, a maraschino cherry, and a shit-load of expensive, high-test liquor. ::

:: Surreptitiously dissolves a Valium or six into the shudder drink. ::

:: Fixes up a nice, filling, drunk-friendly meal. ::

:: Adds another umbrella (and a shot of Everclear) to the frou-frou drink. ::

:: Readies a barf-bucket. ::

:smiley:

You’ll be fine, hon, you know that, yes? :slight_smile:

Where do I send my resume?
I have to admit, I haven’t had much professional experience, but I’m eager to learn. As for qualifications, I can speak in a vaguely foreignish, menacing accent on cue. I have been in labs before and know not to mix chemical A with chemical B just to see what happens. I do not have an unfortunate tendency to fall in love with the hero at the last moment and betray the person who is providing my wages and health plan. Plus, my high heels never break at inopportune moments - they stay high. Really high.

I can start immediately.

Hrrm I have the feeling that my sex underlings will outnumber my monkey army :slight_smile:

Anyhoo, between the nanopure techs and the 2 month long fight with Apple computer and fighting for a replacement of 1500$ of reagents I’ve had enoguh.
Apple computer: My boss paid for an extension on a warrenty, apple cashed the check for it then refused to honor it. I’m still trying to sort out where that check went and if we’re getting our money back…

The reagents: Some elisa kits were delivered and sat in a warehouse for 4 days (90degree weather) and were useless… It took forever to get them replaced :frowning:

But not too deeply. Can’t be too careful, considering the condition of his lab.

SEXY UNDERLINGS! Arrg stupid stupid typos!
Anyhoo, I managed to get a new nanopure machine to be purchased!

WHOOT NEW TOY!

In case you were wondering, “ohms” is a unit of electrical resistance. As I’m sure you know already, impurities such as salts will increase the conductivity of water and measuring the resistance gives you a good approximation of how pure it is. High resistance (ohms)/ low conductivity = good.

As to all the other stuff, I’m an ex-biotech guy and feel your pain. If you want, I could probably look up the sequence for luciferase so as to give the monkey peni the proper firefly effect.

Hehe Beeblebrox :slight_smile:

I already have luciferase virus generated, all I need is a list of the cell recepters exclusive to baboon sexual organs so I can target the virus to one specific part of their bodies :slight_smile:

Fuck using virii, hell, on second thought fuck using luciferase, too. Too damn complicated. Green Flourescent Protein (GHP) injected subcutaneously - that’s the way to go.

GFP is toxic to mamalian cells I thought.

We’ve been having a hell of a time getting our GPF transformed cell line stable, they keep kicking out the plasmid or dying on us :frowning:

Yep, you’re right that it’s probably not the way to go. I was under the mistaken impression that it had been done before in mice. Turns out what I was thinking of was a microinjection of the gene for GFB into the embyos. Some French guy made a rabbit the same way. I’ve never worked with the stuff personally, but the mice and the rabbit had to express the gene, so I am unsure if GFP is toxic in mammals. At any rate, GFP only glows under a black light so it wouldn’t work.

Oh well. Can’t help with the cell receptors for baboons, either, but my old boss might be able to hunt down the receptors for beagles. I know they can’t carry HIV, but I don’t think your enemies would particularly enjoy being raped by a large pack of glow in the dark, exploding beagles.

Um, GFP isn’t even remotely toxic to mammalian cells - and yes, there are a wide variety of mice expressing it all over the place. I work with one of the women who has been part of making such mice. I don’t know what the problem with your cell line is - do you know if they are actually kicking out the plasmid, or just downregulating it? It’s possible that you are not using one of the GFP versions optimized for mammalian expression, or that you’re getting methylation due to high copy number. I know that it is possible to get good GFP expression in human, mouse, hamster, and insect cells. The real problem is, as Beeblebrox pointed out, that you have to shine blue lasers on them to get them to glow.

How about using a ubiquitous virus with a luciferase transgene driven by a genital-specific promoter? And, by all means, buy a new water purification system. Or you can get reagent-grade water by the gallon from several suppliers.

Good luck!