[QUOTE=Vlad/Igor]
Experience goes a long way. I can include in my list of skillZ:
-clinical chemistry, hematology, body fluid analysis, coagulation analysis, toxicology
-Chromatography:
TLC
HPLC
Affinity
size exclusion
-Cell culture
-PCR/RFLP/pharmacogenetic testing
-Immunochemistry
ELISA/ELISA design from just an antibody/antigen pair
Automated
Soon to be added: rtPCR, SELDI mass spec, Western blots
I have not done much on my own, in fact nothing before a paper I’m rewriting that I submitted in June. But, I have skills and experience that would be of great value to a PI. I feel confident that I would not have a hard time finding a job if I were to look for one, based on my experience and skills alone. Educational level is really only looked at for pay scale and general classification (and qualification) of job types; what you can do and are willing to learn are far more important for someone who needs to generate data for the next grant or grant renewal.
Granted you can’t do PhD or advanced MS level work, however I think you have learned more than you are giving yourself credit. My reason for going for an MS in Biochemistry came from the realization that I was training PhDs in some lab techniques and they were asking me for advice on the dynamics of analytical techniques I was very familiar with. What I’m doing now is backfilling my experience with background book knowledge. I’m able to have much deeper discussions about techniques and strategies as a result, and it is very gratifying to have that kind of respect from the PIs and post-docs.
Bench experience and sweat equity do count for something, perhaps half of the value of a lab tech, with formal education being the other half. Plus, if you go into an interview all mealy mouthed about how you don’t have a degree, they’re not going to be interested. Take an honest inventory of what you can do, and present that with confidence to someone who needs your skills, and you’ll have a much better chance at a job. At this point, getting your BS will not only show a level of knowledge, it will also show them that you set a goal and you acheive it, without quitting. This is of great comfort to someone who has a grant to worry about, or needs data for a grant application.
Vlad/Igor
[/QUOTE]
Wow, rtPCR is the hottest thing right now. Its very expensive. Kudoes to you for learning how to do it! (The project I’m working on right now, I have come to believe that PCR stands for Pretty Confusing Results.)
Sounds like you have a lot of great lab-fu! I have been trying to learn various things. Here are the skills I have on my resume:
·Proficient and comfortable handling medium and small animals
·Familiar with mouse colony maintenance, including: weaning pups, determining the sex of animals, blood collection by ocular dislocation and tail bleeding, tail clipping, subcutaneous and inter-peritoneal injections
·Experienced with euthanasia using a carbon dioxide chamber, cervical dislocation and injection of euthanasia solution.
·Trained for non-survival rodent surgery for removing organs and other tissues
·DNA extraction, gel electrophoresis, PCR, flow cytometry, plasmid preparation and tissue culture
·Good communication skills with co-workers, guests and vendors
·Computer literate, comfortable using Microsoft word processing programs, Excel and Power Point
When I started this thread, I was feeling very depressed. For the past year, I have been stuck in a project that is just not working and I’m not getting the suvivial surgery training I hoped for. (The lack of surgery training is on me. I don’t want to harass Rob the Aussie - mouse surgeon master. He’s got piles of work and deadlines to meet. I really, really want to learn from him, but I don’t want to add to his work load.)
I also wonder if my career is a dead end. Right now I’m a Professional Research Assistant/Lab manager. In a few more years, I could apply for a Senior PRA job. That’s it. I’m trying to decide if I’m OK with that. (Another Stepford Student moment, “Yeah. I’ll work as a PRA if I need to re-apply to med school.” Great, my career is someone else’s back up plan. :rolleyes: ) Actually, the PRA holding pattern for med/nursing school is very common. I have seen many people do this. It makes me wonder if there is more I want to do.