Scripted, but not fictional. I first heard it when I was less than ten. It still makes my hair stand on end and my blood run cold.
The first lines of Lawrence Olivier’s narration of The World at War
“Since the beginning of time, man has yearned to destroy the sun.”
- Montgomery Burns, The Simpsons, the episode Who Shot Mr. Burns?
From The Wire, Season 3, Episode 5 (“Straight and True”), spoken by Stringer Bell to one of his underlings:
“Is you taking notes on a criminal fucking conspiracy?”
“Just the one, dear?”
(AbFab S1 Ep2 “Fat”- Eddie’s mother on Eddie trotting out the old idea that “inside me is a thin person trying to get out”)
I remember one scene of Frewer’s character asking a couple of other people if they were talking about him. He then strained his voice to a coarse grunt and said “I’m not paranoid. What did you say?”
That reminds me of my favorite BoJack line: “Now if you’ll excuse me, I need to go take a shower so I can’t tell if I’m crying or not.”
The big reveal on the final episode of Newhart.
Bob Hartley: Well, I was an innkeeper in this crazy little town in Vermont. Nothing made sense in this place. I mean, the maid was an heiress. Her husband talked in alliteration. The handyman kept missing the point of things. And then there were these three woodsmen, but only one of them talked.
Emily Hartley: That settles it, no more Japanese food before you go to bed.
Christopher Walken is master of the memorable line-reading. In an episode from early 2000 (accounts differ–number may be 26/20), he is interacting with “The Love-uhs” (from a recurring sketch in which Will Farrell and Rachel Dratch play ultra-pretentious counter-culture types). There’s a lot of pretentious posing from the pair, and Walken’s companion walks out. His line:
(It’s his delivery that’s so funny.)
“The only reason I went to that orgy was that it was a benefit for charity.” - Nina Van Horn from “Just Shoot Me”.
Exactly my first thought
“Get a life” – William Shatner to Trekkies in an SNL skit.
“Get a life” – William Shatner to Trekkies in an SNL skit.
“Have you ever.. kissed a girl?”
“Get out of your parents’ basements!”
“I wanna be an architect.”
“People underestimate Bob at their peril.”
Justified, Season 4.
For some context: Bob, an overexcited rent-a-cop, is played by Patton Oswalt and is about as intimidating as, well, Patton Oswalt. Everybody on the show spends the entire season underestimating him - until he’s captured by a very nasty bad guy and is brutally tortured, yet not only refuses to fold and give him the information he wants, but instead turns the tables and kills his captor. He then joins up with Raylan, the hero, for his standoff against a much larger group of gangsters. The head gangster looks at little Bob, battered and bloody yet determined, and asks how this guy could kill his top henchman, at which point Raylan, in complete seriousness, says the quoted line. And then the gangsters back off.
There are many great lines from Monty Python!
From the TOS episode “A Private Little War”:
Nona: There is a tradition among my people. When a woman saves a man’s life, he is grateful.
Kirk: I am … grateful.
Delivered like only Shatner can! 
Oh, shut up and go and change your armour. 
(Directed toward Sir Robin. 
)
This.
My very first notion.
From F Troop:
Sgt O’Rourke: Agarn, I don’t know why everybody says you’re so dumb!
Cpl Agarn: (Hours later) Who says I’m dumb? 