The best movie line I read is from American Pie:
“At one time, in band camp, I put a flute in my pussy.”
Infamy, infamy, they’ve all got it infamy. Kenneth Williams, Carry on Cleo.
What did you expect, destroyers? Robert Redford, A Bridge Too Far (to his men grumbling about the flimsy canvas boats in which they’re expected to assault the Rhine)
Afghanistan banana stand. Redford again, The Hot Rock (context: a phrase used to hypnotise a bank guard)
Star Wars, The Empire Strikes Back, Return of the Jedi: “It’s not my fault!” and “I have a bad feeling about this”…
Conan the Barbarian: (Valeria) “Ya wanna live forever?”
Kirk, in **Star Trek II[/]:
“I don’t like to lose.”
Munny, in Unforgiven:
“If anyone touches him, I’ll come back here and kill every single one of you.”
“I’m a mushroom cloud layin’ motherfucker, motherfucker!” - Jules Winfield (Samuel L. Jackson) “Pulp Fiction”
-Syko
“My cat’s breath smells like cat food.” - Ralph Wiggum
More Clint Eastwood:
Midget: “What’d you say your name was, stranger?”
CE: “I didn’t”
CE:“My mistake, 4 coffins”
CE:“Works on about anything, huh? How’s it work on stains?”
Eastwood movies have a ton of them.
Another favorite, but dark–Martin Sheen in Apocalypse Now:
“I told you not to stop, now let’s go.”
Oracle: Would you like a cookie?
…from the Matrix. 
Anita Miller: FECK YOU!
Elaine Miller: HEY!
Anita Miller: This is a house of lies!
Elaine Miller: Well, there it is. Your sister used the “F” word.
William Miller: I think she said “feck.”
Elaine Miller: What’s the difference?
William Miller: The letter “u.”
Jeff Bebe: It’s like a voice that says “Here I am… and fuck you if you can’t understand me.”
William Miller: What do you love about music?
Russell Hammond: To begin with… everything.
-Almost Famous
From The Day the Earth Stood Still, during the demonstration of alien power, when they have shut down all power on Earth. A rich-gent type is having his secretary call the power company to find out why the electricity went out, the secretary tells him the phones are out, he replies, “Well, call the telephone company then.”
And, of course …
** Pop, safty pin, pop. **
Cool Hand Luke“Give us the girl, or we’ll take her”
Conan: <looks around, thinks a bit> “Enough talk.” <throws dagger and kills guard.
- Conan the Destroyer
Love that line.
“There’s nothing worse than an old queen with a head cold” -Victor/Victoria
“I’ve got a touch of hangover Bureaucrat, don’t push me” - McKlintoch
“Just let me have a little bit of peril!” and “A spanking, a spanking!!” - Monty Python’s Search for the Holy Grail
I have no idea why I love this line, but it’s possibly because of the delivery of it in the movie, Steel Magnolias:
“I saw Drum Eatonton the other day, and I smiled at the son-of-a-bitch before I could help myself!” - Ouiser Boudreaux.
From The Graduate:
Benjamin: Wood?
Mrs. Robinson: I beg your pardon.
Benjamin: Wood or wire, they have both.
Mr. Braddock: : Don’t you think that idea is a little half-baked?
Benjamin: Oh no, Dad, it’s completely baked.
Mr. McGuire: I just want to say one word to you… just one word.
Benjamin Braddock: Yes, sir.
Mr. McGuire: Are you listening?
Benjamin Braddock: Yes, sir I am.
Mr. McGuire: “Plastics.”
From Star Wars:
Princess Leia: [Pushing past Chewbacca.] Will someone get this big walking carpet out of my way?