Definitely the mom from The Family Circus. No question about it.
Betty Rubble.
Oh yeah, Joan Jetsen definitely counts as a MILF.
What is a “milf” ?
“Mother I’d Like to Fuck”
Marge Simpson or Lois Griffin
Mother I’d Like to F**K.
Sorry, that should be Jane Jetson.
You had me going with that Joan Jetson remark…I’ve got “I love rock and roll” stuck in my head now. (Joan Jett & the Blackhearts IIRC)
But yeah, Janey would do and Betty too.
I know if I ever get over to “Dogpatch” I’m gonna see if I can hookup with that Daisy Mae. She’s definitely HOT…maybe not a mom as in MILF, either way she’s on my ten most wanted list in cartoon characters. 
we’re kinda pitiful ain’t we
Lois Griffin. Also the mom from Dexter’s Lab, she’s got an ass on her.
I certainly wouldn’t kick Nancy Gribble out of bed. Or her neighbor Minh Supanusanphone…
Lois Griffin.
Betty Rubble always had my vote.
Another vote for Dexter’s mom. Power Thighs.
For Pete’s sake, out of the entire tooniverse three people have chosen Lois Griffin? Oh, I see here that she comes from a wealthy family.
Manjula Nahasapeemapetilon is the hottest cartoon mother of eight I can think of.
Blondie is hot.
Animated- Lois Griffin or Betty Rubble for sure.
Comic strip- Blondie Bumpstead & her friend Trixie (hmmmm! 3-some!), maybe Sally Forth
There are fine single chicks in Apt 3-G tho! Also with Rex Morgan MD.
hey! How is it we’re forgetting Joan in Doonesbury!? Also, isn’t Boopsie a Mom by now?
I wonder what Sailor Moon’s mom looks like?
Lois Griffin. Hot, kinky, rich, and she likes big fat guys. What more could a guy want?
The only downside is Peter would kick my ass.
I understand Mary Jane Watson is in the running now…
Well, you people are in luck. Virtually every MILF listed is the subject of at least a few dozen porn “fanimations.”